《植物生理学报》 2017, 53(4): 581-590

低温胁迫下蒙古黄芪差异表达基因分析

华梦艺^*, 顾卓雅^*, 殷甘强, 王伟, 南蓬^**

复旦大学生命科学学院, 上海200438

通信作者：华梦艺；E-mail: nanpeng@fudan.edu.cn

摘　要：

温度是调控植物生长发育和代谢产物积累的重要因素。为了研究温度调控蒙古黄芪次生代谢产物积累的分子机制, 本文基于转录组测序结果对常温和低温组的蒙古黄芪幼苗基因表达水平进行对比分析。结果表明: 相对于常温组, 低温组差异表达基因共3 896个, 其中表达上调和下调的基因数目分别为2 420和1 476个。关键基因差异表达分析显示, 低温组异黄酮代谢途径中大部分关键基因表达量都显著上调, 而植物激素合成转导途径中的关键基因表达模式各有不同。由此可见, 低温胁迫能够促进蒙古黄芪中部分药用成分的合成, 同时能改变部分内源植物激素的合成和转导, 从而使其更好的适应低温环境。

关键词：蒙古黄芪; 低温胁迫; 异黄酮; 植物内源激素; 差异表达基因

收稿：2016-12-26   修定：2016-03-10

资助：国家自然科学基金(8137963)。

Analysis of differentially expressed gene profile under chilling stress in Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao

HUA Meng-Yi^*, GU Zhuo-Ya^*, YIN Gan-Qiang, WANG Wei, NAN Peng^**

School of Life Sciences, Fudan University, Shanghai 200438, China

Corresponding author: HUA Meng-Yi; E-mail: nanpeng@fudan.edu.cn

Abstract:

Temperature plays an important role in regulating the growth and development of plants as well as the accumulation of metabolites. To investigated the molecular mechanism of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao under chilling stress, we analyzed the seedlings under normal/low temperature treatment by high-throughput transcriptome sequencing. A total of 3 896 differentially expressed genes (DEGs) were screened out in low-temperature group compared to the normal-temperature group, among which 2 420 genes were up-regulated and 1 476 genes were down-regulated. The DEGs involved in the isoflavonids metabolic pathways were all up-regulated, indicating that the synthesis of isoflavonids increased in low temperature. Besides, the phytohormone relative genes showed diversed regulation variation under chilling stress, suggesting various functions of phytohormone in responding chilling stress. Taken together, our study found that the low-temperature stimulus can promote the synthesis of some pharmaceutical components in A. mongolicus and alter the expression profile of phytohormone to adapt to chilling environment.

Key words: Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao; chilling stress; isoflavonids; phytohormone; differentially expressed genes